Biology 3210:

Experimental Methods in Molecular and Cellular Biology

Laboratory Website

Fall 2018

Contacts:

Coordinator

Instructors

Quintin Steynen – steyqj@uleth.ca

Lab Sections and Instructors


Laboratory Materials:

General

Laboratory Manual

SEM Usage Summary

SEM Schedule


Assignment #1 – SEM

Assignment #2 – Restriction Mapping

Assignment #3 – Pseudoscience/Ethics

Assignment #4 – GMO Detection


Lab Report Guidelines

Over-Expression Worksheet

pET28a Information

Science Toolkit - Scientific method and writing resource


Experimental Results

Exercise

Lab 1

Lab 2

R.E. Mapping (wk1)

973, 974, 975, 976, 977, 978, 979, 980, 981, 982, 983, 984, 985, 986, 987, 988, 989, 990

991, 992, 993, 994, 995, 996, 997, 998, 999, 1000, 1001, 1002, 1003

R.E. Mapping (wk2)

1004, 1005, 1006, 1007, 1008, 1009, 1010, 1011, 1012, 1014, 1015, 1016, 1017, 1018, 1019, 1020, 1021

1022, 1023, 1024, 1025, 1026, 1027, 1028, 1029, 1030, 1031, 1032, 1033, 1034, 1035, 1036, 1037, 1038, 1039, 1040

R.E. Mapping (wk3)

1041, 1042, 1043, 1044, 1045, 1046, 1047, 1048, 1049, 1050, 1051, 1052, 1053, 1054, 1055

1056, 1057, 1058, 1059, 1060, 1061, 1062, 1063, 1064, 1065, 1066, 1067, 1068, 1069, 1070, 1071, 1072

Over-Expression

Gel A, Gel B

Gel C, Gel D

Western Blots

Blot A, Blot B

Post_Transfer Gel 1

Post-Transfer Gel 2

Blot C, Blot D

Post_Transfer Gel 3

Post-Transfer Gel 4

GMO Detection

Gel A, Gel B, Gel C, Gel D,

Gel E&F, loading guide

Gel G, Gel H, Gel I, Gel J,

Gel K&L, loading guide