Photomicrographs from Biology 3200 Laboratory Exercise 3
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Gram Stain Endospore Stain Acid Fast Stain
Bacillus thuringiensis B. thuringiensis - Brightfield Mycobacterium smegmatis
Escherichia coli B. thuringiensis - Phase contrast Escherichia coli
Serratia marcescens  
Staphylococcus epidermidis PHB Stain Modified Gin Stain
  B. thuringiensis - Brightfield B. thuringiensis
  B. thuringiensis - Phase contrast Escherichia coli


Title: Gram stain of Escherichia coli cells

Description: Cells were cultivated Trypticase Soy broth for 24 h at 37C. A heat fixed smear was stained with crystal violet and Gram's iodine, decolorized and counterstained with safranin. The slide was observed under brightfield microscopy with a 100 X oil immersion objective.

Material preparation by: Sam Stevenson/Nicole Stady